Riboflavin (E101)
Type of additive (Glossary) | Colorants, Fortifying Agents |
E Number | E101 (iii) |
Chemical Formula | C17H20N4O6 |
Also Known As | Vitamin B2 Lactoflavin |

Purpose and Function
Riboflavin, commonly known as Vitamin B2, is an essential water-soluble vitamin that plays a key role in maintaining energy levels, supporting metabolism, and promoting overall health. In the context of food additives, riboflavin serves several important functions:
- Colorant: Riboflavin is used as a natural colorant in the food industry, imparting a yellow to orange hue to various products. It is utilized in baked goods, cereals, dairy products, sauces, and beverages. Its natural origin and color make it an appealing alternative to synthetic dyes.
- Fortifying Agent: As a fortifying agent, it is added to foods to enhance their nutritional value. It is commonly included in fortified cereals, bread, and baby foods to help prevent vitamin B2 deficiencies in the population. It is particularly beneficial for individuals with restricted diets, such as vegans or those with limited access to a diverse diet.
- Dietary Supplement: Beyond its role as a food additive, riboflavin is also available as a dietary supplement to support overall health. It is crucial for energy production, proper cellular function, and the metabolism of fats, proteins, and carbohydrates.
Riboflavin is necessary for the formation of two important coenzymes, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are vital for various biochemical reactions in the body. It is naturally found in foods such as eggs, green leafy vegetables, milk, meat, and nuts.
Potential Risks and Side Effects
Riboflavin is generally regarded as safe and essential for human health. However, there are a few considerations to keep in mind:
- Toxicity: Riboflavin has a low toxicity risk because it is water-soluble, meaning excess amounts are typically excreted in the urine rather than stored in the body. Therefore, even at high doses, it is unlikely to cause harm. However, extremely large doses, far exceeding typical dietary intake, may cause mild side effects such as diarrhea or an increased risk of kidney stones.
- Yellow Urine: A harmless but notable side effect of supplementation or high intake is bright yellow urine. This occurs because riboflavin is water-soluble and any excess is excreted through the kidneys, giving the urine a distinctive yellow color.
- Photosensitivity: In rare cases, excessive intake could potentially increase sensitivity to light, leading to discomfort in bright environments. This is generally not a concern at typical dietary levels.
- Potential Allergies: Though rare, some individuals may experience allergic reactions to riboflavin when it is used as a food additive. Symptoms may include itching, swelling, or rash, particularly in individuals with pre-existing sensitivities.
Overall, riboflavin is considered safe for the general population and is necessary for maintaining good health. It is recommended to consume riboflavin within the established dietary reference intake levels.